12 4 Lipoprotein lipase is an enzyme found in different tissues including the brain. (a) The enzyme lipoprotein lipase is involved in the metabolism of lipids in the brain. The effect of temperature on lipid metabolism in the brain tissue of a species of fish was investigated. The results of this investigation are shown in the table. Temperature / °C Lipoprotein lipase mRNA / a.u. Triglyceride concentration / mmol mg−1 Fatty acid concentration / µmol mg−1 Lipoprotein lipase activity per milligram of tissue / a.u. 5 4.50 0.58 0.84 1.70 17 1.00 0.60 0.69 1.55 30 1.10 0.70 0.64 0.99 Comment on the effect of temperature on lipid metabolism in this species of fish. 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Turn over 13 (b) Lipoprotein lipase can be extracted from brain tissue to investigate the effect of temperature on the rate of reaction of this enzyme. (i) State what is meant by the term Q10 temperature coefficient. (1) .................................................................................................................................................................................................................................................................................... .................................................................................................................................................................................................................................................................................... .................................................................................................................................................................................................................................................................................... (ii) Devise an investigation to determine Q10 for an extract of lipoprotein lipase enzyme. 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(Total for Question 4 = 9 marks)