Page 02 SECTION 1 — 20 marks Attempt ALL questions 1. The diagram below shows part of a DNA molecule before and after a mutation. T A T A C G C G A T A T G C G C C G C G A T C G T A T A T A T A G C G C mutation before after The type of mutation shown is A deletion B substitution C insertion D inversion. 2. Which of the following are required in a polymerase chain reaction (PCR)? A DNA polymerase, template strand and primers B RNA polymerase, template strand and primers C DNA polymerase, template strand and ligase D RNA polymerase, ligase and primers 3. Each cycle of a polymerase chain reaction (PCR) takes 5 minutes. If there are 1000 DNA fragments at the start of the reaction, how long will it take for the number of fragments produced by the reaction to be greater than 1 million? A 15 minutes B 35 minutes C 50 minutes D 55 minutes