page 10 13. The Gila monster (Heloderma suspectum) is a species of lizard that lives in North America. Its internal temperature is dependent on the environmental temperature. Which row in the table identifies the metabolic cost to and the range of ecological niches of the Gila monster? Metabolic cost Range of ecological niches A low narrow B high narrow C low wide D high wide 14. Which statement describes an event that occurs during the lag phase of microbial growth? A Secondary metabolites are produced B Certain enzymes are induced C Most rapid growth occurs due to plentiful nutrients D The culture medium becomes depleted of nutrients 15. Using recombinant DNA technology, a culture of the bacteria species Micrococcus luteus was genetically modified with a plasmid containing a gene for a human protein. The protein was synthesised by the genetically modified bacteria, but it failed to fold correctly. Which of the following changes to this procedure may lead to a correctly folded protein being produced? A Use a different species of bacteria B Use yeast cells rather than bacteria C Insert an artificial chromosome instead of a plasmid D Insert a regulatory sequence into the plasmid